Effective wound healing on diabetic mice by adhesive antibacterial GNPs-lysine composited hydrogel
Sureerat Khunmanee,
Anseo Choi,
Il Young Ahn,
Woo Ju Kim,
Tae Hui Bae,
Shin Hyuk Kang,
Hansoo Park
Affiliations
Sureerat Khunmanee
Department of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, South Korea
Anseo Choi
Department of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, South Korea
Il Young Ahn
Department of Plastic and Reconstructive Surgery, Chung-Ang University Hospital, Chung-Ang University College of Medicine, 102 Heukseok-ro, Dongjak-gu 06973, South Korea
Woo Ju Kim
Department of Plastic and Reconstructive Surgery, Chung-Ang University Gwangmyeong Hospital, Deokan-ro, Gwangmyeong-si, Gyeonggi-do 14353, South Korea
Tae Hui Bae
Department of Plastic and Reconstructive Surgery, Chung-Ang University Gwangmyeong Hospital, Deokan-ro, Gwangmyeong-si, Gyeonggi-do 14353, South Korea
Shin Hyuk Kang
Department of Plastic and Reconstructive Surgery, Chung-Ang University Hospital, Chung-Ang University College of Medicine, 102 Heukseok-ro, Dongjak-gu 06973, South Korea; Corresponding author
Hansoo Park
Department of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, South Korea; Corresponding author
Summary: Current trends in wound care research focus on creating dressings for diverse wound types, aiming to effectively control the wound healing process. We proposed a wound dressing composed of oxidized hyaluronic acid and amine gelatin with embedded lysine-modified gelatin nanoparticles (HGel-GNPs-lysine). This dressing improves mechanical properties and reduces degradation rates. The storage modulus for HGel-GNPs-lysine was 3,800 Pa, exceeding that of HGel (1,750 Pa). The positively charged surface of GNPs-lysine effectively eliminated Escherichia coli and Staphylococcus aureus. In a diabetic mice model (C57BL/6), HGel-GNPs-lysine immobilized with basic-fibroblast growth factor promoted granulation tissue thickness and collagen density. Gene expression analysis indicated that HGel-GNPs-lysine reduced inflammation and enhanced angiogenesis. This study highlights that HGel-GNPs-lysine could offer alternative treatment strategies for regulating the inflammatory response at the injury site in wound dressing applications.
