Regression Analysis in Development of Method for Isolation and Quantitative Determination of Water-soluble Polysaccharides from Sunflower Roots of One-year-old

Abstract

Introduction. The roots of one-year-old sunflower (Helianthus annuus L.) are mainly wastes of agricultural enterprises when cultivating this crop. At the same time, they differ in a rich chemical composition and contain water-soluble polysaccharides, the base of which is inulin.Aim. The aim of this study is to select optimal parameters of ultrasonic extraction of water-soluble polysaccharides from sunflower roots of oneyear old based on regression analysis.Materials and methods. For the experiment, raw materials purchased from one of the pharmacies in the city of Voronezh were used. A sample of raw materials (1.0 grams) was placed in an ultrasonic bath Grad 40-35, which allows maintaining the necessary temperature and frequency of ultrasound with a given duration of time. Then, 10 (15 or 20) ml of purified water purified from impurities at a temperature of 60 °C to 80 °C was measured with a measuring flask and extracted from 10 to 20 minutes with a multiplicity of 1 to 3, and after each of the multiple studies, the same raw material was further filtered with a new solvent.Results and discussion. The initial conditions and the data obtained during the experiments made it possible to present the regression model in the form of a linear multiple regression equation, which can be used with sufficient accuracy in prediction and analysis. The adequacy of the model was confirmed by testing hypotheses against Pearson's χ2 criterion. The correlation coefficient derived from the values of the regression coefficient and the mean square deviation was used in determining the tightness of the overall relationship between the source and the obtained data. In addition, a detailed study of a sample of those variables whose priority was not clearly expressed was carried out by processing when fixing their values.Conclusion. The most important conditions for extraction of the roots of sunflower of a one-year old should be considered: extraction temperature equal to 80 °C; extraction multiplicity equal to 3; an ultrasound frequency of 35 kHz; Note here that raw material grinding can vary from 0.5 mm to 2 mm, extraction time can range from 15 min to 20 min, ratio of raw material and extractant can vary from 1 g per 10 ml to 1 g per 15 ml.

Keywords

• water-soluble polysaccharides
• inulin
• ultrasonic bath
• roots of one-year-old sunflower
• helianthus annuus l.