Curcumin and Silymarin Amelioration of the Bisphenol-A induced Hepatotoxic Effects in Male Albino Rats: A structural, Biochemical and Molecular Experimental Study

Abstract

Background: Bisphenol A [BPA] is widely used in industrial processes. It persists in environment and exerts a harmful action on human health, through endocrine-disruption. Many natural compounds are suggested to have a preventive effect on BPA-induced human health hazards.Aim of the work: This study aimed to evaluate the effect of Silymarin/Curcumin in ameliorating the hepatotoxic effect induced in albino rats following oral administration of BPA.Patients and Methods: Fourty adult male albino rats were included and randomly categorized into four equal groups. The control group and BPA-exposed [50 mg/kg] group. Groups 3 and 4 for rats received oral BPA [50 mg/kg] plus [100 mg/ kg] of curcumin or silymarin. Serum liver enzymes, pro-inflammatory cytokines [TNF-α & IL-1,6,8] and the oxidant/antioxidant profile were assessed in the serum. SOD, GPX and CAT levels, MDA and H2O2 were assessed. Histopathological changes of the liver, immunohistochemical detection of caspase 3 and area density of collagen fibres were also assessed.Results: Bisphenol-A administration for 30 days was associated with a significant increase in the number of pro-inflammatory markers as interleukin 1,6,8 and tumor necrosis factor‑α [TNF-α]. It also, stimulates oxidative stress with structural changes, apoptosis and fibrosis in hepatic cells. Treatment with curcumin or silymarin for 30 days lead to a reduction of pro‑inflammatory mediators and significant amelioration of structural changes.Conclusion: Silymarin/Curcumin had an ameliorative of harmful effects of BPA on the liver cells through potent anti-inflammatory, antiapoptotic and antioxidant effects.

Keywords

• bisphenol a
• liver, oxidative stress
• hepatoprotective
• silymarin
• curcumin