A Flow SPR Immunosensor Based on a Sandwich Direct Method

Mauro Tomassetti; Giorgia Conta; Luigi Campanella; Gabriele Favero; Gabriella Sanzò; Franco Mazzei; Riccarda Antiochia

doi:10.3390/bios6020022

Biosensors (May 2016)

A Flow SPR Immunosensor Based on a Sandwich Direct Method

Mauro Tomassetti,
Giorgia Conta,
Luigi Campanella,
Gabriele Favero,
Gabriella Sanzò,
Franco Mazzei,
Riccarda Antiochia

Affiliations

Mauro Tomassetti: Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy
Giorgia Conta: Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy
Luigi Campanella: Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy
Gabriele Favero: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy
Gabriella Sanzò: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy
Franco Mazzei: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy
Riccarda Antiochia: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy

DOI: https://doi.org/10.3390/bios6020022
Journal volume & issue: Vol. 6, no. 2
p. 22

Abstract

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In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) methods did not allow the detection of ampicillin. Therefore, a sandwich-based sensor was developed which showed a good linear response towards ampicillin between 10−3 and 10−1 M, a measurement time of ≤20 min and a high selectivity both towards β-lactam antibiotics and antibiotics of different classes.

Published in Biosensors

ISSN: 2079-6374 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.mdpi.com/journal/biosensors

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