Analysis of brain region-specific mRNA synthesis and stability by utilizing adult mouse brain slice culture

Abstract

Summary: Utilization of live animals for mechanistic study is challenging yet pivotal to elucidate pathogenesis of neurological diseases. Here, we present a protocol that employs cultured brain slices derived from adult mice to examine mRNA metabolism. We describe the preparation of acute brain slices and the treatments of RNA synthesis inhibitor and nucleotide analog to examine the effects of ataxin-1 loss-of-function on Bace1 mRNA stability and transcription in cortex. This protocol also includes electrophysiological recording of spontaneous neuronal activity in hippocampus.For complete details on the use and execution of this protocol, please refer to Suh et al. (2019).

Keywords

• Cell Biology
• Genetics
• Gene Expression
• Neuroscience