Fingerprinting alkaloids for traceability: Semi-untargeted UHPLC-MS/MS approach in raw lupins as a case study

Fabiola Eugelio; Sara Palmieri; Marcello Mascini; Francesco Della Valle; Federico Fanti; Eleonora Oliva; Michele Del Carlo; Dario Compagnone; Manuel Sergi

Food Chemistry: X (Oct 2024)

Fingerprinting alkaloids for traceability: Semi-untargeted UHPLC-MS/MS approach in raw lupins as a case study

Fabiola Eugelio,
Sara Palmieri,
Marcello Mascini,
Francesco Della Valle,
Federico Fanti,
Eleonora Oliva,
Michele Del Carlo,
Dario Compagnone,
Manuel Sergi

Affiliations

Fabiola Eugelio: Department of Bioscience and Technologies for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini, 1, 64100 Teramo, TE, Italy
Sara Palmieri: Department of Bioscience and Technologies for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini, 1, 64100 Teramo, TE, Italy
Marcello Mascini: Department of Bioscience and Technologies for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini, 1, 64100 Teramo, TE, Italy
Francesco Della Valle: Department of Bioscience and Technologies for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini, 1, 64100 Teramo, TE, Italy
Federico Fanti: Department of Bioscience and Technologies for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini, 1, 64100 Teramo, TE, Italy; Corresponding author.
Eleonora Oliva: Department of Bioscience and Technologies for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini, 1, 64100 Teramo, TE, Italy
Michele Del Carlo: Department of Bioscience and Technologies for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini, 1, 64100 Teramo, TE, Italy
Dario Compagnone: Department of Bioscience and Technologies for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini, 1, 64100 Teramo, TE, Italy
Manuel Sergi: Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy

Journal volume & issue: Vol. 23
p. 101769

Abstract

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Lupin seeds are recognized for their nutritional value and potential health benefits, but they contain also a significant amount of alkaloids, an anti-nutritive class of compounds, which vary significantly in composition within and between species due to environmental factors. In this study, we developed a predictive multi-experiment approach using ultra-high performance liquid chromatography coupled with triple quadrupole with linear ionic trap tandem mass spectrometry (UHPLC-QqQ-LIT-MS/MS) for comprehensive alkaloid profiling and geographical classification of Lupinus albus L. samples originating from four different Italian regions. Six targeted quinolizidine alkaloids were detected and 21 other alkaloids were putatively identified. Hierarchical Cluster Analysis (HCA) and partial least squares discriminant analysis (PLS-DA) were applied to explore the data structure and successfully classify samples according to their geographical origin. The data demonstrate the efficacy of the developed approach in providing valuable insights in alkaloid profiles of lupin seeds and their potential as markers for geographical traceability.

Published in Food Chemistry: X

ISSN: 2590-1575 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply; Technology: Chemical technology: Food processing and manufacture
Website: https://www.journals.elsevier.com/food-chemistry-x

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