Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī (Feb 2013)
Preventive effects of Cetrorelix on the changes induced by Cisplatin on spermatogenic and myoid cells and basal lamina of seminiferous ducts in the testis of Balb/C mouse
Abstract
Cisplatin used in chemotherapy can have side effect on spermatogenesis. As it is known Gonadotropine hormones (LH, FSH) affect spermatogenesis. It seems by suppressing secretion of LH and FSH during chemotherapy, spermatogenesis could stopped which could result in protection of the cells from cisplatin side effects. The aim of the present study is to investigate the preventive effect of cetrorelix (GnRH antagonist) on spermatogenic defect produced by Cisplatin. In the present study thirty adult male mice aging 6-8 weeks were divided into 3 groups as: Control, Experimental 1 and Experimental 2. Experimental 1 received Cisplatin for 5 days as 2.5 mg/kg intraperitoneally and Experimental 2 received 0.25 mg/kg cetrorelix (GnRH antagonist) one week before cisplatin treatment and continued for 3 weeks. After 35 days from last injection, testicular specimens were removed and prepared for light and electron microscopic study. The data were analyzed using ANOVA (Tukey) in all 3 groups. Histological results showed that nucleus of spermatogonia cells were more condensed and diameter of seminiferous tubules were reduced, in the group which received cisplatin in comparison with the control group. Histochemical studies showed an increase of apoptotic cells in the group which received cisplatin. Electron microscopy showed that in Experimental 1 group basment membrane was morphologically irregular and wavey and Myoid cells were thickened and contracted, but in Experimental 2 group the condition was similar to the control group. These results showed that injection of cetrorelix with cisplatin can protect spermatogenic cells against the side effects of cisplatin.
