The roles of media ingredients in muscle cell culture for cultured meat production—A mini-review
Da Young Lee,
Seung Hyeon Yun,
Juhyun Lee,
Ermie Mariano Jr.,
Yeongwoo Choi,
Dahee Han,
Jinmo Park,
Jin Soo Kim,
Seung Yun Lee,
Sun Jin Hur
Affiliations
Da Young Lee
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea
Seung Hyeon Yun
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea
Juhyun Lee
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea
Ermie Mariano Jr.
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea
Yeongwoo Choi
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea
Dahee Han
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea
Jinmo Park
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea
Jin Soo Kim
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea
Seung Yun Lee
Division of Animal Science, Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, Republic of Korea
Sun Jin Hur
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea; Corresponding author at: Department of Animal Science and Technology, Chung-Ang University, 4726 Seodong-daero, Daedeok-myeon, Anseong-si, Gyeonggi 17456, Republic of Korea.
ABSTRACT: This review was conducted to provide basic knowledge in developing new culture media for cultured meat production by compiling research on how the main media components affect cell proliferation and muscle differentiation. The culturing process can be divided into five processes: cell acquisition, cell proliferation, cell differentiation, myotube formation, and muscle maturation. To date, cultural media research has shown that amino acids, fatty acids, and carbohydrates mainly act as energy and nutrient sources for cell proliferation and muscle production, and minerals mainly play roles as regulators of cell proliferation and muscle production. Vitamins not only protect cells from oxidative stress but also promote cell growth and regulate cell growth-related genes. Additionally, cytokines play a role in regulating genes related to muscle proliferation and regeneration, and hormones, such as growth factors, insulin, and thyroid hormones, benefit muscle growth and regulation.
