DEVELOPMENT OF CREATININE-SENSITIVE BIOSENSOR BASED ON IMMOBILIZED CREATININE DEIMINASE

Abstract

Aim.Thе purpose of the work was to develop a new construction of enzyme biosensor based on creatinine deiminase for highly sensitive creatinine determination. Methods. A new construction of enzyme biosensor based on creatinine deiminase was developed for the creatinine determination. A differential pair of gold interdigitated electrodes deposited onto a ceramic substrate was used as the electrochemical transducer. Creatinine deiminase was immobilized by cross-linking with glutaraldehyde on the surface of electrodes. Results. The biosensor showed high sensitivity towards creatinine, the limit of detection was 5 µM. The biosensor was characterized by wide linear range of creatinine determination, high reproducibility of responses and showed high storage stability – after 50 days storage the biosensor retained 83% of the initial response value. Conclusions. In future the developed biosensor can be used for express evaluation of the creatinine in biological samples.

Keywords

• biosensor
• creatinine
• creatinine deiminase
• creatinine enzymatic assay.