Single cell RNA-seq and ATAC-seq analysis of cardiac progenitor cell transition states and lineage settlement

Guangshuai Jia; Jens Preussner; Xi Chen; Stefan Guenther; Xuejun Yuan; Michail Yekelchyk; Carsten Kuenne; Mario Looso; Yonggang Zhou; Sarah Teichmann; Thomas Braun

doi:10.1038/s41467-018-07307-6

Nature Communications (Nov 2018)

Single cell RNA-seq and ATAC-seq analysis of cardiac progenitor cell transition states and lineage settlement

Guangshuai Jia,
Jens Preussner,
Xi Chen,
Stefan Guenther,
Xuejun Yuan,
Michail Yekelchyk,
Carsten Kuenne,
Mario Looso,
Yonggang Zhou,
Sarah Teichmann,
Thomas Braun

Affiliations

Guangshuai Jia: Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research
Jens Preussner: Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research
Xi Chen: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus
Stefan Guenther: Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research
Xuejun Yuan: Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research
Michail Yekelchyk: Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research
Carsten Kuenne: Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research
Mario Looso: Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research
Yonggang Zhou: Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research
Sarah Teichmann: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus
Thomas Braun: Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research

DOI: https://doi.org/10.1038/s41467-018-07307-6
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 17

Abstract

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Cardiac progenitor cells (CPCs) form cardiomyocytes, pericytes, smooth muscle and endothelial cells during embryonic development. Here, the authors characterize mouse CPCs marked by Nkx2.5 and Isl1 from E7.5 to E9.5 by single cell RNA-seq and ATAC-seq, showing fate transitions involve distinct open chromatin state.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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