The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virus

Abstract

Twenty five isolates of IBV were isolated from 36 broiler and layer chicken farms collected from 13 governorates during 2 years started from January 2003. All the examined farms were vaccinated using the commercial live IB-H120 vaccine in addition to the IB-inactivated vaccine in the layer farm. The viruses were isolated and identified previously by chicken embryo, CEK cell culture inoculation. Isolates subjected to RT-PCR. Four isolates; three broiler farms and one from layer farm were genotyped using S1 partial gene sequencing. Typing of the four isolates using S1 partial gene sequencing, revealed that the isolated IBV strains showed homology to Asia, Europe, USA and Middle East strains.

Keywords

• prevalence; infectious; bronchitis; ib; outbreaks; chicken; farms; molecular; characterization; field; isolates; virus