PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens

Iain J. MacLeod; Christopher F. Rowley; M. Essex

doi:10.1038/s42003-021-01751-9

Communications Biology (Feb 2021)

PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens

Iain J. MacLeod,
Christopher F. Rowley,
M. Essex

Affiliations

Iain J. MacLeod: Department of Immunology and Infectious Diseases, Harvard TH Chan School of Public Health
Christopher F. Rowley: Department of Immunology and Infectious Diseases, Harvard TH Chan School of Public Health
M. Essex: Department of Immunology and Infectious Diseases, Harvard TH Chan School of Public Health

DOI: https://doi.org/10.1038/s42003-021-01751-9
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 13

Abstract

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Iain MacLeod et al. develop the Pan-Degenerate Amplification and Adaptation (PANDAA) point mutation assay to detect HIV drug resistance mutations. This method can be applied to any pathogen where target-proximal genetic variability hinders diagnostic development.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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