Modular detergents tailor the purification and structural analysis of membrane proteins including G-protein coupled receptors

Leonhard H. Urner; Idlir Liko; Hsin-Yung Yen; Kin-Kuan Hoi; Jani Reddy Bolla; Joseph Gault; Fernando Gonçalves Almeida; Marc-Philip Schweder; Denis Shutin; Svenja Ehrmann; Rainer Haag; Carol V. Robinson; Kevin Pagel

doi:10.1038/s41467-020-14424-8

Nature Communications (Jan 2020)

Modular detergents tailor the purification and structural analysis of membrane proteins including G-protein coupled receptors

Leonhard H. Urner,
Idlir Liko,
Hsin-Yung Yen,
Kin-Kuan Hoi,
Jani Reddy Bolla,
Joseph Gault,
Fernando Gonçalves Almeida,
Marc-Philip Schweder,
Denis Shutin,
Svenja Ehrmann,
Rainer Haag,
Carol V. Robinson,
Kevin Pagel

Affiliations

Leonhard H. Urner: Institute of Chemistry and Biochemistry, Freie Universität Berlin
Idlir Liko: Physical and Theoretical Chemistry Laboratory, University of Oxford
Hsin-Yung Yen: Physical and Theoretical Chemistry Laboratory, University of Oxford
Kin-Kuan Hoi: Physical and Theoretical Chemistry Laboratory, University of Oxford
Jani Reddy Bolla: Physical and Theoretical Chemistry Laboratory, University of Oxford
Joseph Gault: Physical and Theoretical Chemistry Laboratory, University of Oxford
Fernando Gonçalves Almeida: OMass Therapeutics, The Schrödinger Building
Marc-Philip Schweder: Institute of Chemistry and Biochemistry, Freie Universität Berlin
Denis Shutin: Physical and Theoretical Chemistry Laboratory, University of Oxford
Svenja Ehrmann: Institute of Chemistry and Biochemistry, Freie Universität Berlin
Rainer Haag: Institute of Chemistry and Biochemistry, Freie Universität Berlin
Carol V. Robinson: Physical and Theoretical Chemistry Laboratory, University of Oxford
Kevin Pagel: Institute of Chemistry and Biochemistry, Freie Universität Berlin

DOI: https://doi.org/10.1038/s41467-020-14424-8
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 10

Abstract

Read online

Detergents are indispensable reagents in membrane protein structural biology. Here, L. H. Urner and co-workers introduce oligoglycerol detergents (OGDs) and use native mass spectrometry to show how interactions of membrane proteins with native membrane lipids can be preserved during purification.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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