Nature Communications (Jan 2020)

Modular detergents tailor the purification and structural analysis of membrane proteins including G-protein coupled receptors

  • Leonhard H. Urner,
  • Idlir Liko,
  • Hsin-Yung Yen,
  • Kin-Kuan Hoi,
  • Jani Reddy Bolla,
  • Joseph Gault,
  • Fernando Gonçalves Almeida,
  • Marc-Philip Schweder,
  • Denis Shutin,
  • Svenja Ehrmann,
  • Rainer Haag,
  • Carol V. Robinson,
  • Kevin Pagel

DOI
https://doi.org/10.1038/s41467-020-14424-8
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 10

Abstract

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Detergents are indispensable reagents in membrane protein structural biology. Here, L. H. Urner and co-workers introduce oligoglycerol detergents (OGDs) and use native mass spectrometry to show how interactions of membrane proteins with native membrane lipids can be preserved during purification.