Bacterial magnetic particles improve testes-mediated transgene efficiency in mice
Chao Wang,
Guanghong Sun,
Ye Wang,
Nana Kong,
Yafei Chi,
Leilei Yang,
Qiliang Xin,
Zhen Teng,
Xu Wang,
Yujun Wen,
Ying Li,
Guoliang Xia
Affiliations
Chao Wang
State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University
Guanghong Sun
State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University
Ye Wang
State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University
Nana Kong
State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University
Yafei Chi
School of Basic Medical Science, Capital Medical University
Leilei Yang
School of Basic Medical Science, Capital Medical University
Qiliang Xin
State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University
Zhen Teng
State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University
Xu Wang
State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University
Yujun Wen
Ningxia Key Laboratory of Cerebrocranial Diseases, Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Ningxia Medical University
Ying Li
State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University
Guoliang Xia
State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University
Nano-scaled materials have been proved to be ideal DNA carriers for transgene. Bacterial magnetic particles (BMPs) help to reduce the toxicity of polyethylenimine (PEI), an efficient gene-transferring agent, and assist tissue transgene ex vivo. Here, the effectiveness of the BMP-PEI complex-conjugated foreign DNAs (BPDs) in promoting testes-mediated gene transfer (TMGT) in mouse was compared with that of liposome-conjugated foreign DNAs. The results proved that through testes injection, the clusters of BPDs successfully reached the cytoplasm and the nuclear of spermatogenesis cell, and expressed in testes of transgene founder mice. Additionally, the ratio of founder mice obtained from BPDs (88%) is about 3 times higher than the control (25%) (p < 0.05). Interestingly, the motility of sperms recovered from epididymis of the founder mice from BPD group were significantly improved, as compared with the control (p < 0.01). Based on classic breeding, the ratio of transgene mice within the first filial was significantly higher in BPDs compared with the control (73.8% versus 11.6%, p < 0.05). TMGT in this study did not produce visible histological changes in the testis. In conclusion, nano-scaled BPDs could be an alternative strategy for efficiently producing transgene mice in vivo.