Generation of Casp8FL122/123GG Mice Using CRISPR-Cas9 Technology

Abstract

Summary: The purpose of this protocol is to describe the generation of missense mutations in mice using CRISPR-Cas9 technology. The current protocol focuses on the generation of a Casp8FL122/123GG missense mutation, but it can be adapted to introduce any missense or nonsense mutation.For complete details on the use and execution of this protocol, please refer to Tummers et al. (2020).

Keywords

• Model Organisms
• CRISPR