Journal of Experimental and Molecular Biology (Mar 2024)

IN VITRO CYTOPHYSIOLOGICAL ASPECTS INDUCED BY FOOD ADDITIVES FROM THE CATEGORY OF PRESERVATIVES ON SOME MAMMALIAN CELL MODELS

  • Daniela Gherghel ,
  • Cosmin-Teodor Mihai,
  • Gabriela Vochita,
  • Craita Maria Rosu

DOI
https://doi.org/10.47743/jemb-2024-149

Abstract

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This research is focused on cellular response to sodium metabisulphite (E223), sodium benzoate (E211), and sodium nitrite (E250) application on two normal mammalian cells, namely MCF-12A (ATCC CRL-3598) and Vero (ATCC CCL-81). The monitored parameters were cell viability (MTT assay), cell morphology (optical microscopy) and cell survival (clonogenic assay). The treatment was applied in doses of 12.5; 25; 50 and 100 µg/mL. According to all tests assessed, our results proved a dose-response relationship, the most sensibility presented the MCF-12A cell line.

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