An Endoscope-like SERS Probe Based on the Focusing Effect of Silica Nanospheres for Tyrosine and Urea Detection in Sweat
Rongyuan Cai,
Lijun Yin,
Qian Huang,
Ruiyun You,
Shangyuan Feng,
Yudong Lu
Affiliations
Rongyuan Cai
Engineering Research Center of Industrial Biocatalysis, Fujian Provincial Key Laboratory of Advanced Oriented Chemical Engineer, Fujian Province Higher Education Institutes, College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, China
Lijun Yin
Engineering Research Center of Industrial Biocatalysis, Fujian Provincial Key Laboratory of Advanced Oriented Chemical Engineer, Fujian Province Higher Education Institutes, College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, China
Qian Huang
Engineering Research Center of Industrial Biocatalysis, Fujian Provincial Key Laboratory of Advanced Oriented Chemical Engineer, Fujian Province Higher Education Institutes, College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, China
Ruiyun You
Engineering Research Center of Industrial Biocatalysis, Fujian Provincial Key Laboratory of Advanced Oriented Chemical Engineer, Fujian Province Higher Education Institutes, College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, China
Shangyuan Feng
Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou 350007, China
Yudong Lu
Engineering Research Center of Industrial Biocatalysis, Fujian Provincial Key Laboratory of Advanced Oriented Chemical Engineer, Fujian Province Higher Education Institutes, College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, China
In this work, we developed a new type of SERS probe, which was composed of glass-SiO2-Au@MBN@Ag nanoparticles (NPs) three-dimensional Surface-enhanced Raman spectroscopy (SERS) substrate. When the laser passed through the quartz glass sheet, on the one hand, the SiO2 NPs supporting the Au@MBN@Ag NPs increase the roughness of the substrate surface, resulting in a large number of hot spots among nanoparticles. On the other hand, based on the focusing effect of silicon dioxide nanospheres, the laser can better focus on the surface of nanoparticles in the inverted SERS probe, thus showing better SERS enhancement. Furthermore, the Au@MBN@Ag NPs core-shell structure was used with 4-mercaptobenzoonitrile (MBN) as an internal standard molecule, and the quantitative determination of tyrosine and urea was realized by internal standard correction method. The standard working curves of the two had good linear correlation with R2 above 0.9555. The detection limits of tyrosine and urea were in the range of 2.85 × 10−10 M~7.54 × 10−6 M, which confirms that this design can be used for quantitative and specific detection of biological molecules, demonstrating great practical significance for the research of diseases such as skin lesions and endocrine disorders.
