Optical-Spectrometry-Based Method for Immunosuppressant Medicine Level Detection in Aqueous Solutions
Marcin Marzejon,
Monika Kosowska,
Daria Majchrowicz,
Barbara Bułło-Piontecka,
Michał Wąsowicz,
Małgorzata Jędrzejewska-Szczerska
Affiliations
Marcin Marzejon
Department of Metrology and Optoelectronics, Faculty of Electronics, Telecommunications and Informatics, Gdańsk University of Technology, 11/12 Narutowicza Street, 80-233 Gdansk, Poland
Monika Kosowska
Department of Metrology and Optoelectronics, Faculty of Electronics, Telecommunications and Informatics, Gdańsk University of Technology, 11/12 Narutowicza Street, 80-233 Gdansk, Poland
Daria Majchrowicz
Department of Metrology and Optoelectronics, Faculty of Electronics, Telecommunications and Informatics, Gdańsk University of Technology, 11/12 Narutowicza Street, 80-233 Gdansk, Poland
Barbara Bułło-Piontecka
Department of Nephrology, Transplantology and Internal Diseases, Faculty of Medicine, Medical University of Gdańsk, 3a Marii Skłodowskiej-Curie Street, 80-210 Gdansk, Poland
Michał Wąsowicz
Department of Morphological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 159 Nowoursynowska Street, 02-776 Warszawa, Poland
Małgorzata Jędrzejewska-Szczerska
Department of Metrology and Optoelectronics, Faculty of Electronics, Telecommunications and Informatics, Gdańsk University of Technology, 11/12 Narutowicza Street, 80-233 Gdansk, Poland
In this paper, an investigation into detecting immunosuppressive medicine in aqueous solutions using a spectrometry-based technique is described. Using optical transmissive spectrometry, absorbance measurements in the spectra range from 250 nm to 1000 nm were carried out for different cyclosporine A (CsA) concentrations in aqueous solutions. The experiment was conducted for samples both with and without interferent substances—glucose and sodium chloride. Using a dedicated algorithm, the measured data was analyzed and a high correlation coefficient R2 = 0.8647 was achieved. The experiment showed that the described technique allowed for the detection of various CsA concentration levels in a selective, label-free and simple way. This method could be used in medicine, veterinary medicine and laboratory diagnostics.
