Nature Communications (May 2020)
Computational stabilization of T cell receptors allows pairing with antibodies to form bispecifics
- Karen Froning,
- Jack Maguire,
- Arlene Sereno,
- Flora Huang,
- Shawn Chang,
- Kenneth Weichert,
- Anton J. Frommelt,
- Jessica Dong,
- Xiufeng Wu,
- Heather Austin,
- Elaine M. Conner,
- Jonathan R. Fitchett,
- Aik Roy Heng,
- Deepa Balasubramaniam,
- Mark T. Hilgers,
- Brian Kuhlman,
- Stephen J. Demarest
Affiliations
- Karen Froning
- Eli Lilly Biotechnology Center, 10300 Campus Point Drive
- Jack Maguire
- Program in Bioinformatics and Computational Biology, University of North Carolina at Chapel Hill
- Arlene Sereno
- Eli Lilly Biotechnology Center, 10300 Campus Point Drive
- Flora Huang
- Eli Lilly Biotechnology Center, 10300 Campus Point Drive
- Shawn Chang
- Eli Lilly Biotechnology Center, 10300 Campus Point Drive
- Kenneth Weichert
- Eli Lilly Biotechnology Center, 10300 Campus Point Drive
- Anton J. Frommelt
- Eli Lilly Biotechnology Center, 10300 Campus Point Drive
- Jessica Dong
- Eli Lilly Biotechnology Center, 10300 Campus Point Drive
- Xiufeng Wu
- Eli Lilly Biotechnology Center, 10300 Campus Point Drive
- Heather Austin
- Eli Lilly Biotechnology Center, 10300 Campus Point Drive
- Elaine M. Conner
- Eli Lilly Biotechnology Center, 10300 Campus Point Drive
- Jonathan R. Fitchett
- Eli Lilly Biotechnology Center, 10300 Campus Point Drive
- Aik Roy Heng
- Eli Lilly Biotechnology Center, 10300 Campus Point Drive
- Deepa Balasubramaniam
- Eli Lilly Biotechnology Center, 10300 Campus Point Drive
- Mark T. Hilgers
- Eli Lilly Biotechnology Center, 10300 Campus Point Drive
- Brian Kuhlman
- Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill
- Stephen J. Demarest
- Eli Lilly Biotechnology Center, 10300 Campus Point Drive
- DOI
- https://doi.org/10.1038/s41467-020-16231-7
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 14
Abstract
Recombinant T-cells receptors can redirect naïve T cells but often have low or uneven expression. Here, the authors model mutations in TCR constant domains to increase T cell receptor expression, assembly, and stability and generate bispecific molecules.
