Characterization of flavonoids in Millettia nitida var. hirsutissima by HPLC/DAD/ESI-MSn

Min Ye; Wen-Zhi Yang; Ke-Di Liu; Xue Qiao; Bei-Jia Li; Jun Cheng; Jie Feng; De-An Guo; Yu-Ying Zhao

Journal of Pharmaceutical Analysis (Feb 2012)

Characterization of flavonoids in Millettia nitida var. hirsutissima by HPLC/DAD/ESI-MSn

Min Ye,
Wen-Zhi Yang,
Ke-Di Liu,
Xue Qiao,
Bei-Jia Li,
Jun Cheng,
Jie Feng,
De-An Guo,
Yu-Ying Zhao

Affiliations

Min Ye: Corresponding authors. Tel.: +86 10 8280 1516; fax: +86 10 8280 2024.; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China
Wen-Zhi Yang: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China
Ke-Di Liu: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China
Xue Qiao: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China
Bei-Jia Li: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China
Jun Cheng: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China
Jie Feng: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China
De-An Guo: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China
Yu-Ying Zhao: Corresponding authors. Tel.: +86 10 8280 1516; fax: +86 10 8280 2024.; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China

Journal volume & issue: Vol. 2, no. 1
pp. 35 – 42

Abstract

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Millettia nitida var. hirsutissima is a Chinese herbal medicine used for the treatment of gynecological diseases. An HPLC/DAD/ESI-MSn method was established for the rapid separation and characterization of bioactive flavonoids in M. nitida var. hirsutissima. A total of 32 flavonoids were detected, of which 14 compounds were unambiguously characterized by comparing their retention time, UV, and MS spectra with those of the reference standards, and the others were tentatively identified based on their tandem mass spectrometry fragmentation data obtained in the negative ionization mode on line. Nineteen of these compounds characterized were reported from this plant for the first time. Keywords: HPLC/DAD/ESI-MSn, Millettia nitida var. hirsutissima, Mass spectrometry, Flavonoids

Published in Journal of Pharmaceutical Analysis

ISSN: 2095-1779 (Print); 2214-0883 (Online)
Publisher: Elsevier
Country of publisher: China
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/journal-of-pharmaceutical-analysis/

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