Evaluating Helicobacter Pylori FlaA in Clinical Isolates from Alzahra Hospital, Isfahan, Iran

Abstract

Background: This study aimed to survey the presence of flagellin gene flaA in isolated Helicobacter pylori. Methods: One hundred gastric biopsies were collected by a gastroenterologist and inoculated in enrichment medium under microaerophilic conditions. DNA was extracted and polymerase chain reaction (PCR) was performed using the designed primer. Findings: PCR products showed that all extracted DNA were positive for flaA gene. Conclusion: We found flaA gene to be present in all clinical isolates. As it is highly similar to standard Helicobacter pylori strains, it can be used to detect the mentioned bacteria.

Keywords

• FlaA
• Helicobacter pylori