Keratin extraction optimization from poultry feather using response surface- Box-Behnken experimental design method

Abstract

This study aimed to develop a cost-effective method for managing chicken feather waste by hydrolyzing it in an alkaline medium to extract keratin hydrolysate. Through a Box-Behnken experimental design, the effects of NaOH solution concentration, temperature, and extraction time on the extraction process were examined. Analysis of the extracted keratin hydrolysate revealed the presence of key functional groups and characteristic protein structures. Optimization efforts identified 0.65 N NaOH solution concentration, 70 °C temperature, and 1-h extraction time as yielding the highest protein yield of 89.71 %. These findings underscore the potential of alkaline hydrolysis as a sustainable approach for both waste management and the extraction of valuable keratin hydrolysate. This research contributes to addressing the challenges faced by poultry farms in waste management while offering avenues for the utilization of keratin in various applications, thereby promoting environmental protection and human health.

Keywords

• Alkaline hydrolysis
• Circular economy
• Keratin filler
• Keratin protein
• Poultry feature