Phagocytic Activity of Neutrophilic Granulocytes in Response to the Effect of Extended-Spectrum /3-Lactamases (Staphylococcus aureus; Klebsiella pneumoniae; Pseudomonas aeruginosa)

Abstract

The study investigates the phagocytic activity of neutrophilic granulocytes under the influence of antibiotic-resistant strains of bacteria Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiellapneimoniae belonging to extended-spectrum в-lactamases. The subjects of the study were neutrophilic granulocytes of blood isolated from healthy people and strains of bacteria S.aureus, P.aeruginosa, and K.pneumoniae resistant and sensitive to antibiotics. The functions of phagocytosis (phagocytic number and phagocytic index) were assessed with the help of FITC (Fluorescein Isothyocyanate) - labeled bacteria. The analysis of stained cells was carried out on flow cytofluorimeter FC-500 (BeckmanCoulter, USA) in whole peripheral blood. A different phagocytic response of neutrophils to resistant and sensitive strains of bacteria was detected. Thus, in response to resistant strains of S.aureus, the percentage of neutrophils entering phagocytosis and the average number of intracellular bacteria increases with respect to sensitive strains. In response to resistant strains of K.pneumonia, the phagocytic activity of neutrophilic granulocytes decreases. When neutrophil granulocytes were induced, resistant strains of P.aeruginosa did not show significant differences with respect to sensitive strains.

Keywords

• klebsiella pneumoniae
• staphylococcus aureus
• pseudomonas aeruginosa
• antibiotic resistance
• neutrophilic granulocytes
• phagocytosis
• klebsiella pneumoniae
• staphylococcus aureus
• pseudomonas aeruginosa