Mode of Bioenergetic Metabolism during B Cell Differentiation in the Intestine Determines the Distinct Requirement for Vitamin B1
Jun Kunisawa,
Yuki Sugiura,
Taichi Wake,
Takahiro Nagatake,
Hidehiko Suzuki,
Risa Nagasawa,
Shiori Shikata,
Kurara Honda,
Eri Hashimoto,
Yuji Suzuki,
Mitsutoshi Setou,
Makoto Suematsu,
Hiroshi Kiyono
Affiliations
Jun Kunisawa
Laboratory of Vaccine Materials, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, Japan
Yuki Sugiura
Department of Biochemistry, Keio University School of Medicine, and Japan Science and Technology Agency (JST), Exploratory Research for Advanced Technology (ERATO), Suematsu Gas Biology Project, Tokyo 160-8582, Japan
Taichi Wake
Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
Takahiro Nagatake
Laboratory of Vaccine Materials, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, Japan
Hidehiko Suzuki
Laboratory of Vaccine Materials, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, Japan
Risa Nagasawa
Laboratory of Vaccine Materials, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, Japan
Shiori Shikata
Laboratory of Vaccine Materials, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, Japan
Kurara Honda
Department of Biochemistry, Keio University School of Medicine, and Japan Science and Technology Agency (JST), Exploratory Research for Advanced Technology (ERATO), Suematsu Gas Biology Project, Tokyo 160-8582, Japan
Eri Hashimoto
Laboratory of Vaccine Materials, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, Japan
Yuji Suzuki
Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
Mitsutoshi Setou
Department of Cell Biology and Anatomy and Medical Photonics Research Center System Molecular Anatomy, Hamamatsu University School of Medicine, Shizuoka 431-3192, Japan
Makoto Suematsu
Department of Biochemistry, Keio University School of Medicine, and Japan Science and Technology Agency (JST), Exploratory Research for Advanced Technology (ERATO), Suematsu Gas Biology Project, Tokyo 160-8582, Japan
Hiroshi Kiyono
Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
Bioenergetic metabolism varies during cell differentiation, but details of B cell metabolism remain unclear. Here, we show the metabolic changes during B cell differentiation in the intestine, where B cells differentiate into IgA+ plasma cells (PCs). Naive B cells in the Peyer’s patches (PPs) and IgA+ PCs in the intestinal lamina propria (iLP) both used the tricarboxylic acid (TCA) cycle, but only IgA+ PCs underwent glycolysis. These metabolic differences reflected their dependencies on vitamin B1, an essential cofactor for the TCA cycle. Indeed, the diminished activity of the TCA cycle after dietary vitamin B1 depletion decreased the number of naive B cells in PPs without affecting IgA+ PCs in the iLP. The maintenance of naive B cells by dietary vitamin B1 was required to induce—but not maintain—intestinal IgA responses against oral antigens. These findings reveal the diet-mediated maintenance of B cell immunometabolism in organized and diffuse intestinal tissues.
