Differential gene expression analysis and SNP/InDel marker discovery in resistant wild Asparagus kiusianus and susceptible A. officinalis in response to Phomopsis asparagi infection
Mostafa Abdelrahman,
Mai Mitoma,
Takao Ikeuchi,
Mitsutaka Mori,
Kyoko Murakami,
Yukio Ozaki,
Masaru Matsumoto,
Atsuko Uragami,
Akira Kanno
Affiliations
Mostafa Abdelrahman
Arid Land Research Center, Tottori University, Tottori 680-0001, Japan; Botany Department, Faculty of Sciences, Aswan University, Aswan 81528, Egypt; Correspondence to: Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan.
Mai Mitoma
Arid Land Research Center, Tottori University, Tottori 680-0001, Japan
This data article reports de novo transcriptome analysis of resistant wild Asparagus kiusianus and susceptible A. officinalis plants 24 and 48 h post-inoculation (24 and 48 hpi) with Phomopsis asparagi. Differential gene expression (DGE) analysis demonstrated that several genes involved in secondary metabolites and plant-pathogen interactions are up-regulated in resistant wild A. kiusianus relative to susceptible A. officinalis. The assembled contig sequences generated in this study were used to search single nucleotide polymorphism (SNP) and insertion/deletion (InDel) distribution in A. kiusianus and A. officinalis plants. SNP and InDel data developed from this transcriptome analysis will be used to generate a high-density linkage map to facilitate further development of molecular marker-assisted selection in A. officinalis. Keywords: RNA-Seq, Transcriptome, Asparagus, Single nucleotide polymorphism, Insertion/deletion
