Pollen Competition and Paternal Contribution during Artificially Controlled Pollination of Black Locust (<i>Robinia pseudoacacia</i> L.) without Castration
Yuhan Sun,
Ruiyang Hu,
Li Dong,
Xiuyu Li,
Zijie Zhang,
Qi Guo,
Sen Cao,
Jiankang Li,
Peiyao Han,
Chao Han,
Saleem Uddin,
Cui Long,
Yingming Fan,
Yun Li
Affiliations
Yuhan Sun
Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
Ruiyang Hu
Experimental Center of Forestry in North China, Chinese Academy of Forestry, National Permanent Scientific Research Base for Warm Temperate Zone Forestry of Jiulong Mountain in Beijing, Beijing 102300, China
Li Dong
Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
Xiuyu Li
Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
Zijie Zhang
Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
Qi Guo
Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
Sen Cao
Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
Jiankang Li
Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
Peiyao Han
Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
Chao Han
Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
Saleem Uddin
Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
Cui Long
Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
Yingming Fan
Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
Yun Li
Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
(1) Background: Considering the serious damage caused by castration and the extremely high outcrossing rate in nature, we hypothesized that artificial controlled pollination of black locust without castration could be conducted for hybridization breeding. (2) Methods: The study conducted controlled pollination on 20 mating combinations of black locust without castration using a single or mixed male parent. Offspring of different developmental stages and the leaves of parents were collected to extract DNA and perform paternity analysis using SSR molecular markers. (3) Results: The contribution rate of each male parent differed according to developmental stage after pollination using different pollens mixed in equal proportions. There were significant correlations between the genetic similarity between each male parent and female parent and contribution rate of each male parent at three different developmental stages after pollination. (4) Conclusions: The composition of offspring pollen donors showed no bias toward selfing or outcrossing when artificially pollinated without castration. Hybrid breeding of black locust by artificially controlled pollination without castration may not be feasible, given that our manual method resulted in a large number of abortive and abnormal offspring. Introduction of honeybees in a limited space to conduct controlled pollination of black locust for hybrid breeding may be feasible.
