Expression profile analysis of muscle regulation genes under growth and water flow stress in zebrafish

Xiudan Yuan; Leiting Tao; Xiaoli Hu; Ruoyu Lin; Jingping Yang; Mengzhe Feng; Mei Peng; Wenbin Liu; Yamei Xiao

Reproduction and Breeding (Mar 2024)

Expression profile analysis of muscle regulation genes under growth and water flow stress in zebrafish

Xiudan Yuan,
Leiting Tao,
Xiaoli Hu,
Ruoyu Lin,
Jingping Yang,
Mengzhe Feng,
Mei Peng,
Wenbin Liu,
Yamei Xiao

Affiliations

Xiudan Yuan: State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, Hunan, 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha, 410081, China; College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, China
Leiting Tao: State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, Hunan, 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha, 410081, China; College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, China
Xiaoli Hu: State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, Hunan, 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha, 410081, China; College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, China
Ruoyu Lin: State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, Hunan, 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha, 410081, China; College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, China
Jingping Yang: College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, China
Mengzhe Feng: College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, China
Mei Peng: College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, China
Wenbin Liu: State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, Hunan, 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha, 410081, China; College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, China; Corresponding author. State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, 410081, China.
Yamei Xiao: State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, Hunan, 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha, 410081, China; College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, China; Corresponding author. State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, 410081, China.

Journal volume & issue: Vol. 4, no. 1
pp. 5 – 9

Abstract

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Muscle is one of the important quality of fish. However, there is still a lack of sufficient understanding of the mechanism of muscle growth regulation in fish. In this study, the expression patterns of 10 reported muscle development related genes are analyzed in zebrafish during growth or under water flow stress. Myogenic regulatory factors (MyoD, MyoG, MRF4), myostatin regulatory genes (Mstna, Mstnb) and myoblast development-related genes (Pax7, Prmt5, Desmin, MYHC) are significantly increased expression at somite stage and muscle effect stage embryos in zebrafish. The mRNA levels of the muscle development related genes are up-regulated in 7-day fries and 30-day juvenile of zebrafish. And there are differences in the expression levels of seven muscle development related genes (MyoD, Myf5, MyoG, Mstna, Mstnb, Desmin, MYHC) between the large and small individuals of 20-day-old zebrafish. Results indicated that short-term water flow stressing can promote zebrafish muscle growth, while prolonged water flow stress may lead to motor fatigue and affect growth in zebrafish. This research provides a foundation for further exploration of the growth regulation mechanism in fish, and is helpful on fish farming.

Published in Reproduction and Breeding

ISSN: 2667-0712 (Online)
Publisher: KeAi Communications Co. Ltd.
Country of publisher: China
LCC subjects: Science: Biology (General): Genetics; Science: Biology (General): Reproduction; Science: Physiology: Animal biochemistry
Website: https://www.keaipublishing.com/en/journals/reproduction-and-breeding/

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