Effect of Aqueous Extract of Ferula Assa-Foetida and Avicennia Marina on Oxidative Stress Parameters of Brian Cells in Diabetic Rats

Abstract

Introduction Increased insulin resistance, inflammatory factors, and oxidative stress parameters are associated with the development of diabetes complications. The aim of this study was to evaluate the effect of Ferula assa-foetida and Avicennia marina aqueous extracts on the activity of antioxidant enzymes of brain cells in diabetic rats. Materials and Methods In this experimental study, 54 male rats were randomly divided into the equal groups of control, diabetic control and experimental diabetic 1 and 2. Experimental diabetic and diabetic control groups became diabetes by an intraperitoneal injection of 120 mg/kg alloxan. The experimental diabetic groups received the aqueous extract of Ferula assa-foetida (100 and 200 mg/kg, ip) and Avicennia marina (100 and 200 mg/kg, ip) in alternate days for one month. Sterile distilled water was injected to the control and diabetic control groups. On 1st, 15th and 30th day after induction of experimental diabetes, the levels of superoxide dismutase (SOD), glutathione s-transferase (GST), catalase (CAT) antioxidant enzymes and malondialdehyde (MDA) level were measured in the brian cells. Data analyzed by two way repeated measures ANOVA and post-hoc LSD test. Results Injection of Ferula assa-foetida 200 mg/kg, ip) and Avicennia marina extracts 200 mg/kg, ip) to the diabetic rats resulted in a significant decrease in the MDA levels and significant increases in the level of SOD, GST, CAT enzymes in brian cells (P<0.05). Conclusion The results of this study confirms the dose-dependent antioxidant role of Ferula assa-foetida and Avicennia marina extracts on improving the oxidative stress in the brain cells of diabetic rats.

Keywords

• Enzymes
• Antioxidant
• Brian
• Diabetes
• Rats