Metallomic study of mercury in fish from Amazon region - Brazil

Abstract

This paper presents preliminary findings for a metallomics study of mercury in the muscle of the fish from Amazonas region – Brazil, after protein separation by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and subsequent evaluation of mercury by Thermal Decomposition and Amalgamation Coupled with Atomic Absorption (TTA-CAAS). It was found that mercury is present in 18 protein spots of “dourada” muscle (Brachyplatystoma rousseauxii) and 9 protein spots of “pacu” muscle (Myleussp.). The protein spots in which they were determined the presence of mercury present molecular weight of 13.5 and 21.4 kDa and isoelectric point of 3.8 and 9.2, respectively.

Keywords

• Mercury in fish
• Metallomic study
• 2D-PAGE
• TTA-CAAS