Molecular Analysis of Streptomycin Resistance Genes in Clinical Strains of <i>Mycobacterium tuberculosis</i> and Biocomputational Analysis of the <i>Mt</i>GidB L101F Variant
Álvaro Rodríguez-García,
Rosa E. Mares-Alejandre,
Patricia L. A. Muñoz-Muñoz,
Samuel Ruvalcaba-Ruiz,
Ricardo A. González-Sánchez,
Johanna Bernáldez-Sarabia,
Samuel G. Meléndez-López,
Alexei F. Licea-Navarro,
Marco A. Ramos-Ibarra
Affiliations
Álvaro Rodríguez-García
Biotechnology and Biosciences Research Group, Faculty of Chemical Sciences and Engineering, Autonomous University of Baja California, Tijuana 22390, Mexico
Rosa E. Mares-Alejandre
Biotechnology and Biosciences Research Group, Faculty of Chemical Sciences and Engineering, Autonomous University of Baja California, Tijuana 22390, Mexico
Patricia L. A. Muñoz-Muñoz
Biotechnology and Biosciences Research Group, Faculty of Chemical Sciences and Engineering, Autonomous University of Baja California, Tijuana 22390, Mexico
Samuel Ruvalcaba-Ruiz
Clinical Diagnostic Laboratory, General Hospital of Tijuana, Tijuana 22010, Mexico
Ricardo A. González-Sánchez
Department of Biomedical Innovation, Center for Scientific Research and Higher Education at Ensenada, Ensenada 22860, Mexico
Johanna Bernáldez-Sarabia
Department of Biomedical Innovation, Center for Scientific Research and Higher Education at Ensenada, Ensenada 22860, Mexico
Samuel G. Meléndez-López
Biotechnology and Biosciences Research Group, Faculty of Chemical Sciences and Engineering, Autonomous University of Baja California, Tijuana 22390, Mexico
Alexei F. Licea-Navarro
Department of Biomedical Innovation, Center for Scientific Research and Higher Education at Ensenada, Ensenada 22860, Mexico
Marco A. Ramos-Ibarra
Biotechnology and Biosciences Research Group, Faculty of Chemical Sciences and Engineering, Autonomous University of Baja California, Tijuana 22390, Mexico
Globally, tuberculosis (TB) remains a prevalent threat to public health. In 2019, TB affected 10 million people and caused 1.4 million deaths. The major challenge for controlling this infectious disease is the emergence and spread of drug-resistant Mycobacterium tuberculosis, the causative agent of TB. The antibiotic streptomycin is not a current first-line anti-TB drug. However, WHO recommends its use in patients infected with a streptomycin-sensitive strain. Several mutations in the M. tuberculosisrpsL, rrs and gidB genes have proved association with streptomycin resistance. In this study, we performed a molecular analysis of these genes in clinical isolates to determine the prevalence of known or novel mutations. Here, we describe the genetic analysis outcome. Furthermore, a biocomputational analysis of the MtGidB L101F variant, the product of a novel mutation detected in gidB during molecular analysis, is also reported as a theoretical approach to study the apparent genotype-phenotype association.
