Antibiotics (Jul 2021)

Molecular Analysis of Streptomycin Resistance Genes in Clinical Strains of <i>Mycobacterium tuberculosis</i> and Biocomputational Analysis of the <i>Mt</i>GidB L101F Variant

  • Álvaro Rodríguez-García,
  • Rosa E. Mares-Alejandre,
  • Patricia L. A. Muñoz-Muñoz,
  • Samuel Ruvalcaba-Ruiz,
  • Ricardo A. González-Sánchez,
  • Johanna Bernáldez-Sarabia,
  • Samuel G. Meléndez-López,
  • Alexei F. Licea-Navarro,
  • Marco A. Ramos-Ibarra

DOI
https://doi.org/10.3390/antibiotics10070807
Journal volume & issue
Vol. 10, no. 7
p. 807

Abstract

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Globally, tuberculosis (TB) remains a prevalent threat to public health. In 2019, TB affected 10 million people and caused 1.4 million deaths. The major challenge for controlling this infectious disease is the emergence and spread of drug-resistant Mycobacterium tuberculosis, the causative agent of TB. The antibiotic streptomycin is not a current first-line anti-TB drug. However, WHO recommends its use in patients infected with a streptomycin-sensitive strain. Several mutations in the M. tuberculosisrpsL, rrs and gidB genes have proved association with streptomycin resistance. In this study, we performed a molecular analysis of these genes in clinical isolates to determine the prevalence of known or novel mutations. Here, we describe the genetic analysis outcome. Furthermore, a biocomputational analysis of the MtGidB L101F variant, the product of a novel mutation detected in gidB during molecular analysis, is also reported as a theoretical approach to study the apparent genotype-phenotype association.

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