The Application of eDNA for Monitoring Aquatic Non-Indigenous Species: Practical and Policy Considerations

Abstract

Aquatic non-indigenous species (NIS) threaten biodiversity, ecosystem functions, and the economy worldwide. Monitoring NIS is of immediate concern to identify newly arriving species, assess the efficacy of mitigation measures, and report long-term indicators of introduction, spread, and impacts. The challenges associated with conventional methods of specimen collection and morphological identification have led to the development of alternative methods, such as DNA-based methods, which could offer rapid and cost-effective detection of NIS. Depending on whether a few (targeted monitoring) or many species (passive monitoring) are being monitored, environmental DNA (eDNA) can infer presence-absence and relative abundances, enabling informed decisions and actions to be made based on patterns of detection. Compared to more conventional methods, eDNA tools can increase the levels of detection and sensitivity for rare and elusive species, which is even more noticeable for some taxa when using targeted monitoring. The use of DNA-based tools not only minimizes the onus on taxonomic expertise and reduces resource demands but can also be more sensitive and cost-efficient in detecting NIS, thus proving its value as an early warning tool. As nucleic acid (DNA/RNA) methods advance rapidly for NIS detection, there must be a balance between method sensitivity, logistical requirements, and associated costs, which must be factored into future management decisions. While there are many complementary reviews available, our aim is to emphasize the importance of incorporating eDNA tools into NIS surveys and to highlight the available opportunities in this field.

Keywords

• non-native species
• NIS
• eDNA
• targeted-passive monitoring
• eRNA
• HTS