Mitochondrial fission is associated with UCP1 activity in human brite/beige adipocytes

Didier F. Pisani; Valentin Barquissau; Jean-Claude Chambard; Diane Beuzelin; Rayane A. Ghandour; Maude Giroud; Aline Mairal; Sophie Pagnotta; Saverio Cinti; Dominique Langin; Ez-Zoubir Amri

Molecular Metabolism (Jan 2018)

Mitochondrial fission is associated with UCP1 activity in human brite/beige adipocytes

Didier F. Pisani,
Valentin Barquissau,
Jean-Claude Chambard,
Diane Beuzelin,
Rayane A. Ghandour,
Maude Giroud,
Aline Mairal,
Sophie Pagnotta,
Saverio Cinti,
Dominique Langin,
Ez-Zoubir Amri

Affiliations

Didier F. Pisani: Université Côte d'Azur, CNRS, Inserm, iBV, Nice, France; Corresponding author. Institut de Biologie Valrose, Univ. Nice Sophia Antipolis, 28, avenue de Valombrose, 06107 Nice Cedex 2, France. Fax: +33 493 37 70 58.
Valentin Barquissau: Inserm, UMR1048, Obesity Research Laboratory, Institute of Metabolic and Cardiovascular Diseases, Toulouse 31432, France; University of Toulouse, UMR1048, Paul Sabatier University, Toulouse 31432, France
Jean-Claude Chambard: Université Côte d'Azur, CNRS, Inserm, iBV, Nice, France
Diane Beuzelin: Inserm, UMR1048, Obesity Research Laboratory, Institute of Metabolic and Cardiovascular Diseases, Toulouse 31432, France; University of Toulouse, UMR1048, Paul Sabatier University, Toulouse 31432, France
Rayane A. Ghandour: Université Côte d'Azur, CNRS, Inserm, iBV, Nice, France
Maude Giroud: Université Côte d'Azur, CNRS, Inserm, iBV, Nice, France
Aline Mairal: Inserm, UMR1048, Obesity Research Laboratory, Institute of Metabolic and Cardiovascular Diseases, Toulouse 31432, France; University of Toulouse, UMR1048, Paul Sabatier University, Toulouse 31432, France
Sophie Pagnotta: Université Côte d'Azur, CNRS, Inserm, iBV, Nice, France; Centre Commun de Microscopie Appliquée, Microscopy and Imaging Platform Côte d'Azur, Nice 06108, France
Saverio Cinti: Department of Experimental and Clinical Medicine, University of Ancona (Politecnica Delle Marche), Ancona 60121, Italy; Center of Obesity, University of Ancona (Politecnica Delle Marche)–United Hospitals, Ancona 60121, Italy
Dominique Langin: Inserm, UMR1048, Obesity Research Laboratory, Institute of Metabolic and Cardiovascular Diseases, Toulouse 31432, France; University of Toulouse, UMR1048, Paul Sabatier University, Toulouse 31432, France; Toulouse University Hospitals, Department of Clinical Biochemistry, Toulouse 31059, France
Ez-Zoubir Amri: Université Côte d'Azur, CNRS, Inserm, iBV, Nice, France; Corresponding author. Institut de Biologie Valrose, Univ. Nice Sophia Antipolis, 28, avenue de Valombrose, 06107 Nice Cedex 2, France. Fax: +33 493 37 70 58.

Journal volume & issue: Vol. 7
pp. 35 – 44

Abstract

Read online

Objective: Thermogenic adipocytes (i.e. brown or brite/beige adipocytes) are able to burn large amounts of lipids and carbohydrates as a result of highly active mitochondria and enhanced uncoupled respiration, due to UCP1 activity. Although mitochondria are the key organelles for this thermogenic function, limited human data are available. Methods/results: We characterized changes in the mitochondrial function of human brite adipocytes, using hMADS cells as a model of white- to brite-adipocyte conversion. We found that profound molecular modifications were associated with morphological changes in mitochondria. The fission process was partly driven by the DRP1 protein, which also promoted mitochondrial uncoupling. Conclusion: Our data demonstrate that white-to-brite conversion of human adipocytes relies on molecular, morphological and functional changes in mitochondria, which enable brite/beige cells to carry out thermogenesis. Keywords: hMADS, UCP1, DRP1, Brite/beige adipocyte, Mitochondria

Published in Molecular Metabolism

ISSN: 2212-8778 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Internal medicine
Website: https://www.journals.elsevier.com/molecular-metabolism/

About the journal