Quantitative analysis of myofiber type composition in human and mouse skeletal muscles

Abstract

Summary: Skeletal muscles are composed of different myofiber types characterized by the expression of myosin heavy chain isoforms, which can be affected by physical activity, aging, and pathological conditions. Here, we present a step-by-step high-throughput semi-automated approach for performing myofiber type quantification of entire human or mouse muscle tissue sections, including immunofluorescence staining, image acquisition, processing, and quantification.For complete details on the use and execution of this protocol, please refer to Abbassi-Daloii et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• High-Throughput Screening
• Microscopy