Characterization of MicroRNA* Species in Peking Duck Skin

Li ZHANG; Xiu-juan XIE; Shan-gang JIA; Mei XIAO; Shu-dai LIN; Li-long AN; Wen LUO; Xin-zheng JIA; Qing-hua NIE; Xi-quan ZHANG

Journal of Integrative Agriculture (Sep 2013)

Characterization of MicroRNA* Species in Peking Duck Skin

Li ZHANG,
Xiu-juan XIE,
Shan-gang JIA,
Mei XIAO,
Shu-dai LIN,
Li-long AN,
Wen LUO,
Xin-zheng JIA,
Qing-hua NIE,
Xi-quan ZHANG

Affiliations

Li ZHANG: Agricultural College, Guangdong Ocean University, Zhanjiang 524088, P.R. China; ZHANG Li, Correspondence ZHANG Xi-quan, Tel/Fax: +86-20-85285703
Xiu-juan XIE: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R. China
Shan-gang JIA: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, P.R. China
Mei XIAO: Agricultural College, Guangdong Ocean University, Zhanjiang 524088, P.R. China
Shu-dai LIN: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R. China
Li-long AN: Agricultural College, Guangdong Ocean University, Zhanjiang 524088, P.R. China
Wen LUO: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R. China
Xin-zheng JIA: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R. China
Qing-hua NIE: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R. China
Xi-quan ZHANG: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R. China; ZHANG Li, Correspondence ZHANG Xi-quan, Tel/Fax: +86-20-85285703

Journal volume & issue: Vol. 12, no. 9
pp. 1614 – 1619

Abstract

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A substantial fraction of miRNA* species are conserved in animals and can repress activities of target genes. This study aims to investigate the miRNA* species in duck skin by using Solexa sequencing. We obtained a total of 96 miRNA* species in two skin small RNA libraries and identified 56 miRNA/miRNA* (miR/miR*) pairs. Nucleotide bias of miRNA* indicated that the priority was C>A>U>G for the first nucleotide and U>C>A>G for the last nucleotide. Comparison analyses showed that 3′-U accounted for a higher proportion in the 56 miR/miR* pairs. Among the top 20 expressed miRNA* species, 17 were shared by two libraries and most of the miRNA* species were highly conservative, especially in the “seed region”. miR-199a* were expressed highly in our samples, which was also previously shown abundant in mouse hair follicle. Furthermore, four miRNA* species were predicted to target their genes in signal pathways of feather follicle development and feather morphogenesis despite very low levels.

Published in Journal of Integrative Agriculture

ISSN: 2095-3119 (Print); 2352-3425 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.journals.elsevier.com/journal-of-integrative-agriculture

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