Precise tuning of gene expression levels in mammalian cells

Yale S. Michaels; Mike B. Barnkob; Hector Barbosa; Toni A. Baeumler; Mary K. Thompson; Violaine Andre; Huw Colin-York; Marco Fritzsche; Uzi Gileadi; Hilary M. Sheppard; David J. H. F. Knapp; Thomas A. Milne; Vincenzo Cerundolo; Tudor A. Fulga

doi:10.1038/s41467-019-08777-y

Nature Communications (Feb 2019)

Precise tuning of gene expression levels in mammalian cells

Yale S. Michaels,
Mike B. Barnkob,
Hector Barbosa,
Toni A. Baeumler,
Mary K. Thompson,
Violaine Andre,
Huw Colin-York,
Marco Fritzsche,
Uzi Gileadi,
Hilary M. Sheppard,
David J. H. F. Knapp,
Thomas A. Milne,
Vincenzo Cerundolo,
Tudor A. Fulga

Affiliations

Yale S. Michaels: Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Mike B. Barnkob: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine University of Oxford
Hector Barbosa: Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Toni A. Baeumler: Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Mary K. Thompson: Department of Biochemistry, University of Oxford
Violaine Andre: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine University of Oxford
Huw Colin-York: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine University of Oxford
Marco Fritzsche: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine University of Oxford
Uzi Gileadi: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine University of Oxford
Hilary M. Sheppard: School of Biological Sciences, University of Auckland
David J. H. F. Knapp: Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Thomas A. Milne: Weatherall Institute of Molecular Medicine, MRC Molecular Haematology Unit, NIHR Oxford Biomedical Research Centre Programme, University of Oxford
Vincenzo Cerundolo: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine University of Oxford
Tudor A. Fulga: Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford

DOI: https://doi.org/10.1038/s41467-019-08777-y
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 12

Abstract

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Analogue regulation of gene expression is important for normal function in mammals but existing genetic technologies are designed to achieve ON/OFF control. Here the authors develop synthetic microRNA silencing-mediated fine-tuners (miSFITs) to precisely control target gene expression levels.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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