Combined exercise training decreases blood pressure in OLDER women with NOS3 polymorphism providing changes in differentially methylated regions (DMRs)
Guilherme da Silva Rodrigues,
Natalia Yumi Noronha,
João Gabriel Ribeiro de Lima,
Isabela Harumi Yonehara Noma,
Andressa Crystine da Silva Sobrinho,
Luísa Maria Diani,
Ana P. Pinto,
Karine Pereira Rodrigues,
Marcela Augusta de Souza Pinhel,
Carla Barbosa Nonino,
Lígia Moriguchi Watanabe,
Carlos Roberto Bueno Júnior
Affiliations
Guilherme da Silva Rodrigues
Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil
Natalia Yumi Noronha
Department of Gynecology and Obstetrics, University Medical Center Groningen, Groningen, The Netherlands
João Gabriel Ribeiro de Lima
Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil
Isabela Harumi Yonehara Noma
Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil
Andressa Crystine da Silva Sobrinho
Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil
Luísa Maria Diani
Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil
Ana P. Pinto
School of Physical Education of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil
Karine Pereira Rodrigues
Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil
Marcela Augusta de Souza Pinhel
Department of Health Sciences, Ribeirão Preto Medical School, São Paulo, Brazil
Carla Barbosa Nonino
Department of Health Sciences, Ribeirão Preto Medical School, São Paulo, Brazil
Lígia Moriguchi Watanabe
Department of Health Sciences, Ribeirão Preto Medical School, São Paulo, Brazil
Carlos Roberto Bueno Júnior
Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil
The mechanisms by which the ageing process is associated to an unhealthy lifestyle and how they play an essential role in the aetiology of systemic arterial hypertension have not yet been completely elucidated. Our objective is to investigate the influence of NOS3 polymorphisms [−786T > C and (Glu298Asp)] on systolic blood pressure (SBP) and diastolic blood pressure (DBP) response, differentially methylated regions (DMRs), and physical fitness of adult and older women after a 14-week combined training intervention. The combined training was carried out for 14 weeks, performed 3 times a week, totalling 180 minutes weekly. The genotyping experiment used Illumina Infinium Global Screening Array version 2.0 (GSA V2.0) and Illumina’s EPIC Infinium Methylation BeadChip. The participants were separated into SNP rs2070744 in TT (59.7 ± 6.2 years) and TC + CC (60.0 ± 5.2 years), and SNP rs17999 in GluGlu (58.8 ± 5.7 years) and GluAsp + AspAsp (61.6 ± 4.9 years). We observed an effect of time for variables BP, physical capacities, and cholesterol. DMRs related to SBP and DBP were identified for the rs2070744 and rs17999 groups pre- and decreased numbers of DMRs post-training. When we analysed the effect of exercise training in pre- and post-comparisons, the GluGlu SNP (rs17999) showed 10 DMRs, and after enrichment, we identified several biological biases. The combined training improved the SBP and DBP values of the participants regardless of the SNPs. In addition, exercise training affected DNA methylation differently between the groups of NOS3 polymorphisms.
