Antimicrobial and Isolation of Stigmasterol from Ethyl Acetate Extract of Musa Acuminate Calla Flowers

Abstract

Banana blossom is traditionally used in northern Nigeria to treat a variety of ailments, including microbiological infections; reduces menstrual bleeding; aids in the treatment of diabetes, anaemia, and ulcers. Reduces anxiety, aids in weight loss, and produces an undetermined amount of filtrate decoction to facilitate conception. As a result, the study intends to isolate secondary metabolites to identify the bioactive compound(s) present in banana flowers. Based on the antimicrobial activity results, the highest inhibitory concentration for ethyl acetate extracts of the Banana blossom flower is 12.5 mg/ml, as indicated below. Compounds are isolated using column chromatography. Isolation was performed using an ethyl acetate extract of the banana blossom flower. The fractions from the column are subjected to TLC testing to determine the separation of the component combination using a thin stationary phase supported by an inert backing, resulting in BF16 fractions. The goal of TLC is to produce well-defined, well-separated spots. Each component has a retention factor (RF), which joins fractions with equal RF values. We extracted stigmasterol from Musa acuminata calla ethyl acetate extracts and analysed it using NMR spectroscopy and FT-IR.

Keywords

• musa acuminata calla
• antimicrobial activities
• stigmasterol
• extraction