PLoS ONE (Jan 2019)

First clinical isolate of Escherichia coli harboring mcr-1 gene in Mexico.

  • Jocelin Merida-Vieyra,
  • Agustín De Colsa-Ranero,
  • Patricia Arzate-Barbosa,
  • Eduardo Arias-de la Garza,
  • Alfonso Méndez-Tenorio,
  • Jazmin Murcia-Garzón,
  • Alejandra Aquino-Andrade

DOI
https://doi.org/10.1371/journal.pone.0214648
Journal volume & issue
Vol. 14, no. 4
p. e0214648

Abstract

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Our aim in this report was to describe the characteristics of the first clinical isolate of Escherichia coli (EC-PAG-733) harboring the mcr-1 gene found in Mexico. This isolate was obtained from a fecal sample from a young child with an oncological condition. We obtained the whole-genome sequence using next-generation sequencing and analyzed the sequence by bioinformatics tools. EC-PAG-733 was resistant to third- and fourth-generation cephalosporins and was susceptible to all carbapenems and amikacin; it was also resistant to ciprofloxacin, levofloxacin, gentamicin and colistin at a minimum inhibitory concentration (MIC) of 4 μg/mL. This isolate was classified as O11:H25-ST457. EC-PAG-733 harbored an ESBL type CTX-M-55 as well as several virulence factors that have been associated with Enteroaggregative Escherichia coli (EAEC). The mcr-1 gene was located within an IncI2 plasmid. The results of this whole genome shotgun project were deposited in DDBJ/ENA/GenBank under the accession number QKXE00000000.