Expression of Eleven Egg Performance-associated Genes in the Ovary of Zi Geese Anser anser domestica

Abstract

The relative expression of eleven egg performance-associated genes including the tau-crystallin/α enolase (ENO1), ribosomal protein S27a (RPS27A), calmodulin 1 (CALM1), solute carrier family 25 A6 (SLC25A6), arylsulfatase A (ARSA), syndecan 2 (SDC2), ribosomal protein L10a (RPL10A), ornithine decarboxylase antizyme 1 (OAZ1), cytochrome c somatic variant 2 (CYCS), adenosine triphosphate synthase, H+ transporting, α subunit, isoform 1 (ATP5A1) and the ring finger protein 130 (RNF130) in the ovaries of pre-laying and laying Zi geese were analyzed using quantitative real-time PCR (qRT-PCR). Comparison between pre-laying and egg-laying stages, the relative expression of ENO1, RPS27A, SLC25A6, ARSA, SDC2, RPL10A, OAZ1, CYCS and RNF130 were significantly up-regulated (P<0.05), whereas the CALM1 and ATP5A1 were highly significantly up-regulated (P<0.01). This study established the primary foundation to understand the possible roles of these genes in the ovaries.

Keywords

• egg performance-associated genes
• geese
• ovaries
• qrt-pcr