Modern technology advances of Pseudomonas aeruginosa based biosensor approach

Abstract

The threat posed by microbial infections to human health remains very significant, particularly in considering the increasing mechanisms of antibiotic resistance. Pseudomonas aeruginosa is known for its antibiotic resistance and is linked to serious conditions like ventilator-associated pneumonia and cystic fibrosis. Rapid infection elimination relies on identifying harmful bacteria and preventing colonization. Due to the redox-active nature of P. aeruginosa biomarkers metabolites, several electrochemical sensing approaches are being evaluated as diagnostic platforms for the rapid and precise detection of P. aeruginosa with high sensitivity. These sensors must be developed and modified to achieve optimal selectivity, sensitivity, and biocompatibility, enabling dynamic, real-time metabolite detection. This minireview highlights recent advancements in electrochemical and optical biosensors for detecting P. aeruginosa biomarkers, particularly pyocyanin. It highlighted design, analytical performance, biological recognition elements, and personalized wearable electrochemical sensor devices. It concludes with the future suggestions of sensor concepts for clinical diagnostics, environmental monitoring, and food safety.

Keywords

• Electrochemical biosensor
• Optical biosensor
• Biomarker metabolites
• Pseudomonas aeruginosa
• Diagnosis
• Infections