Influence of Pretilachlor on some physiological properties in Chlorella vulgaris

Abstract

Micro algaes have a major role in food chains and aquatic ecosystems. Among the mono cellular algaes in the paddy fields which locted in Mazandaran state, Chlorella vulgaris is an important species in Chlorophyta phylum. Influence of herbicides as the most serious particle of pesticides on the algaes is very considerable. Thererfore this study is performed in orther to determination effect of pertilachlor on the some properties of Chlorella vulgaris. Nine treatments (0, 20, 30, 40, 50, 75, 100, 150 and 300 mg herbicide per 1 liter) prepared in one litter bottle, then 100 cc algae solution and stock culture was added, next this system aerated. Treatments with three replications during 24, 48, 72 and 96 hours were investigated. After treating, effect of pretilachlor on the desity, chlorophyll a concentration, activity of catalase, peroxidase, ascorbat peroxidase, polyphenoloxidase enzymes and lipidic peroxidation were examined. Herbicide increasing at the all times resulted in significant reduction about cellular density (p < 0.0001). Maximum and minimum cellular density was measured in the control (without herbicide) and 300 mg pertilachlor treatment, respectively. The less Chl a consentration was obsereved in the most dose herbicide. Activity rate of quadruple enzymes in long term (9 days), indicated significant loss with increasing herbicide dose. Also lipidic peroxidation in all treatments was more relative to control. In this study was shown enzymes activity in high pretilachlor concentrations was not enough that can prevent the reactive oxygen species and this material was targeted by herbicide.

Keywords

• chlorella
• enzymatic activity
• pretilachlor
• herbicide
• bioenvironment pollution