Fine Mapping the Soybean Mosaic Virus Resistance Gene in Soybean Cultivar Heinong 84 and Development of CAPS Markers for Rapid Identification
Yong Li,
Xinlei Liu,
Wenjia Deng,
Jiahui Liu,
Yue Fang,
Ye Liu,
Tingshuai Ma,
Ying Zhang,
Yongguo Xue,
Xiaofei Tang,
Dan Cao,
Zhifei Zhu,
Xiaoyan Luan,
Xiaofei Cheng
Affiliations
Yong Li
School of Life Science, Northeast Agricultural University, Harbin 150030, China
Xinlei Liu
Soybean Research Institute, Heilongjiang Academy of Agricultural Science, Harbin 150086, China
Wenjia Deng
Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region of Chinese Education Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150030, China
Jiahui Liu
Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region of Chinese Education Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150030, China
Yue Fang
Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region of Chinese Education Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150030, China
Ye Liu
Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region of Chinese Education Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150030, China
Tingshuai Ma
Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region of Chinese Education Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150030, China
Ying Zhang
Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region of Chinese Education Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150030, China
Yongguo Xue
Soybean Research Institute, Heilongjiang Academy of Agricultural Science, Harbin 150086, China
Xiaofei Tang
Soybean Research Institute, Heilongjiang Academy of Agricultural Science, Harbin 150086, China
Dan Cao
Soybean Research Institute, Heilongjiang Academy of Agricultural Science, Harbin 150086, China
Zhifei Zhu
Soybean Research Institute, Heilongjiang Academy of Agricultural Science, Harbin 150086, China
Xiaoyan Luan
Soybean Research Institute, Heilongjiang Academy of Agricultural Science, Harbin 150086, China
Xiaofei Cheng
Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region of Chinese Education Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150030, China
Heinong 84 is one of the major soybean varieties growing in Northeast China, and is resistant to the infection of all strains of soybean mosaic virus (SMV) in the region including the most prevalent strain, N3. However, the resistance gene(s) in Heinong 84 and the resistant mechanism are still elusive. In this study, genetic and next-generation sequencing (NGS)-based bulk segregation analysis (BSA) were performed to map the resistance gene using a segregation population from the cross of Heinong 84 and a susceptible cultivar to strain N3, Zhonghuang 13. Results show that the resistance of Heinong 84 is controlled by a dominant gene on chromosome 13. Further analyses suggest that the resistance gene in Heinong 84 is probably an allele of Rsv1. Finally, two pairs of single-nucleotide-polymorphism (SNP)-based primers that are tightly cosegregated with the resistance gene were designed for rapidly identifying resistant progenies in breeding via the cleaved amplified polymorphic sequence (CAPS) assay.
