Sexual Dimorphism in the Expression of Cardiac and Hippocampal Renin-Angiotensin and Kallikrein–Kinin Systems in Offspring from Mice Exposed to Alcohol during Gestation
Gabriel Almeida da Silva,
Allan Luís Barboza Atum,
Leonardo Paroche de Matos,
Guilherme Rabelo Nasuk,
Bruna Calixto de Jesus,
Telma Luciana Furtado Gouveia,
Ovidiu Constantin Baltatu,
Stella Regina Zamuner,
José Antônio Silva Júnior
Affiliations
Gabriel Almeida da Silva
Medicine Department, Universidade Nove de Julho (UNINOVE), Rua Vergueiro 249, Liberdade, São Paulo 01504-001, SP, Brazil
Allan Luís Barboza Atum
Medicine Department, Universidade Nove de Julho (UNINOVE), Rua Vergueiro 249, Liberdade, São Paulo 01504-001, SP, Brazil
Leonardo Paroche de Matos
Medicine Department, Universidade Nove de Julho (UNINOVE), Rua Vergueiro 249, Liberdade, São Paulo 01504-001, SP, Brazil
Guilherme Rabelo Nasuk
Medicine Department, Universidade Nove de Julho (UNINOVE), Rua Vergueiro 249, Liberdade, São Paulo 01504-001, SP, Brazil
Bruna Calixto de Jesus
Medicine Department, Universidade Nove de Julho (UNINOVE), Rua Vergueiro 249, Liberdade, São Paulo 01504-001, SP, Brazil
Telma Luciana Furtado Gouveia
Odontology Department, Ágora Faculty, Av. Sorrento, Campo Novo do Parecis 78360-000, MT, Brazil
Ovidiu Constantin Baltatu
Center of Innovation, Technology and Education (CITE), Anhembi Morumbi University, Anima Institute, Sao Jose dos Campos Technology Park, Sao Jose dos Campos 12247-016, SP, Brazil
Stella Regina Zamuner
Medicine Department, Universidade Nove de Julho (UNINOVE), Rua Vergueiro 249, Liberdade, São Paulo 01504-001, SP, Brazil
José Antônio Silva Júnior
Medicine Department, Universidade Nove de Julho (UNINOVE), Rua Vergueiro 249, Liberdade, São Paulo 01504-001, SP, Brazil
Prenatal alcohol exposure (PAE) impairs fetal development. Alcohol consumption was shown to modulate the renin–angiotensin system (RAS). This study aimed to analyze the effects of PAE on the expression of the renin–angiotensin system (RAS) and kallikrein–kinin system (KKS) peptide systems in the hippocampus and heart of mice of both sexes. C57Bl/6 mice were exposed to alcohol during pregnancy at a concentration of 10% (v/v). On postnatal day 45 (PN45), mouse hippocampi and left ventricles (LV) were collected and processed for messenger RNA (mRNA) expression of components of the RAS and KKS. In PAE animals, more pronounced expression of AT1 and ACE mRNAs in males and a restored AT2 mRNA expression in females were observed in both tissues. In LV, increased AT2, ACE2, and B2 mRNA expressions were also observed in PAE females. Furthermore, high levels of H2O2 were observed in males from the PAE group in both tissues. Taken together, our results suggest that modulation of the expression of these peptidergic systems in PAE females may make them less susceptible to the effects of alcohol.
