Jichu yixue yu linchuang (Feb 2022)

Hepatocyte-specific Sirt1 deficiency exacerbates liver inflammation of mice

  • YANG Jia-hui, ZHAO Wei, XIE Xiang-hong, LI Chun-mei, LIU Xiao-jun, YAO Hong

DOI
https://doi.org/10.16352/j.issn.1001-6325.2022.02.007
Journal volume & issue
Vol. 42, no. 2
pp. 282 – 285

Abstract

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Objective To investigate the effect of deletion of Sirtuin type 1(Sirt1) gene on liver inflammation of mice and its mechanism. Methods The body weight of C57BL/6J male mice and liver specific Sirt1 deficiency (Sirt1-LKO) male mice were recorded weekly. The sensitivity of inflammatory response was tested by intraperitoneal injection of 20 mg/kg LPS. The survival curve was used to reflect the sensitivity of mice to inflammatory response.The expression of inflammatory factors was detected by Western blot and q-PCR. Results Compared with control wild type mice, Sirt1-LKO mice showed significantly reduced body weight (P＜0.05). In addition, The survival curve was shown by kaplane Meier diagram,Sirt1-LKO mice showed lower sensitivity to LPS (P＜0.05).The protein level of NF-κB in primary liver cells of Sirt1-LKO mice was increased (P＜0.05),Tnfα and IL6 of NF-κB downstream target genes were up-regulated(P＜0.05). Conclusions Hepatocyte-specific Sirt1 deletion aggravates liver inflammation response in mouse model.

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