Communications Biology (Jun 2022)

A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii

  • Yoichiro Ito,
  • Misa Ishigami,
  • Goro Terai,
  • Yasuyuki Nakamura,
  • Noriko Hashiba,
  • Teruyuki Nishi,
  • Hikaru Nakazawa,
  • Tomohisa Hasunuma,
  • Kiyoshi Asai,
  • Mitsuo Umetsu,
  • Jun Ishii,
  • Akihiko Kondo

DOI
https://doi.org/10.1038/s42003-022-03475-w
Journal volume & issue
Vol. 5, no. 1
pp. 1 – 12

Abstract

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A high-throughput workflow involving a streamlined genome-wide screen improves protein production in the non-conventional yeast, Komagataella phaffii, following adaptive laboratory evolution.