Novelty in Biomedicine (Feb 2014)
Effect of Dietary Supplementation with Conjugated Linoleic Acid on Bone Mineral Density, Bone Metabolism Markers and Inflammatory Markers in Healthy Post-menopausal Women: a Randomized Double Blind Placebo Controlled Trial
Abstract
AbstractIntroduction: Conjugated linoleic acid (CLA) has been shown to positively influence on calcium and bone metabolism in experimental animals and cell culture, but there are limited human data available.Material and Methods: This double-blind, placebo-controlled trial study was done on 76 healthy post-menopausal women (aged 55.1) which randomly assigned to receive daily four CLA capsules G80 containing 3.2 g isomer blend (50:50% cis-9, trans-11: trans-10, cis-12 isomers) or four capsules containing high oleic sunflower oil as placebo for 12 weeks. Urine and blood samples were collected at weeks 0 and 12 and were analyzed for biomarkers of calcium and bone metabolism and inflammatory markers (TNF-α and IL-6). Subjects completed 3-day dietary records during the trial, in weeks 0 (baseline), 6 and 12.Results: supplementation with 3.2 g CLA isomer blend (50:50% cis-9,trans-11:trans-10,cis-12 isomers) for 12 weeks had no significant effects on bone formation markers (serum osteocalcin, bone-specific alkaline phosphatase) or bone resorption (urine C-telopeptide-related fraction of type 1 collagen degradation products), parathyroid hormone (PTH), urinary calcium, urinary creatinine and CTP to creatinine ratio. But serum interlukine-6 did not change significantly over 12 weeks in postmenopausal women.Conclusion: Under the conditions tested in postmenopausal women, 3.2 g CLA isomer blend (50:50% cis-9, trans-11: trans-10, cis-12 isomers) did not affect markers of bone metabolism and calcium.
