Autofluorescence-Based Investigation of Spatial Distribution of Phenolic Compounds in Soybeans Using Confocal Laser Microscopy and a High-Resolution Mass Spectrometric Approach
Mayya P. Razgonova,
Yulia N. Zinchenko,
Darya K. Kozak,
Victoria A. Kuznetsova,
Alexander M. Zakharenko,
Sezai Ercisli,
Kirill S. Golokhvast
Affiliations
Mayya P. Razgonova
Far Eastern Experimental Station, N.I. Vavilov All-Russian Institute of Plant Genetic Resources, 190000 Saint-Petersburg, Russia
Yulia N. Zinchenko
Far Eastern Experimental Station, N.I. Vavilov All-Russian Institute of Plant Genetic Resources, 190000 Saint-Petersburg, Russia
Darya K. Kozak
Laboratory of Biochemistry, Blagoveshchensk State Pedagogical University, 675000 Blagoveshchensk, Russia
Victoria A. Kuznetsova
Far Eastern Experimental Station, N.I. Vavilov All-Russian Institute of Plant Genetic Resources, 190000 Saint-Petersburg, Russia
Alexander M. Zakharenko
Laboratory of Pesticide Toxicology, Siberian Federal Scientific Center of Agrobiotechnology RAS, 633501 Krasnoobsk, Russia
Sezai Ercisli
Department of Horticulture, Agricultural Faculty, Ataturk University, Erzurum 25240, Turkey
Kirill S. Golokhvast
Far Eastern Experimental Station, N.I. Vavilov All-Russian Institute of Plant Genetic Resources, 190000 Saint-Petersburg, Russia
In this research, we present a detailed comparative analysis of the bioactive substances of soybean varieties k-11538 (Russia), k-11559 (Russia), k-569 (China), k-5367 (China), k-5373 (China), k-5586 (Sweden), and Primorskaya-86 (Russia) using an LSM 800 confocal laser microscope and an amaZon ion trap SL mass spectrometer. Laser microscopy made it possible to clarify in detail the spatial arrangement of the polyphenolic content of soybeans. Our results revealed that the phenolics of soybean are spatially located mainly in the seed coat and the outer layer of the cotyledon. High-performance liquid chromatography (HPLC) was used in combination with an amaZon SL BRUKER DALTONIKS ion trap (tandem mass spectrometry) to identify target analytes in soybean extracts. The results of initial studies revealed the presence of 63 compounds, and 45 of the target analytes were identified as polyphenolic compounds.
