Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum (Aug 2011)
Culture Compound Effects on the Production of Hyaluronidase Enzyme through Bacillus
Abstract
Background and Objectives: Today, hyaluronidase enzyme has a wide application in medicine, pharmaceutics, histoegineering, oncology and ophthalmology. Its therapeutical significance is based upon the breakage of hyaluronan, resulting in increasing the level of membranous permeability, decreasing viscosity index and facilitating the spread of injected liquids. In fact, hyaluronidase causes a huge increase in the absorption of some injected drugs like antibiotics improving the efficiency of any local anesthetics. Today, producing this kind of enzyme through microorganisms has attracted considerableattention. Considering that BHI broth culture is an expensive medium and cannot be used as an economical medium for industrial production of the enzyme, designing a synthetic culture medium has been considered in order to keep its production within the limit of BHI (7.4unit/ml) in an economical manner.Methods: The isolate G8 (obtained from the soil of Behesht Zahra district in Tehran) was used as a nativestrain and producer of the enzyme .G8 is a kind of aerobic soporiferous bacillus.In addition mall compounds contained in the culture were recognized and their concentrations were measured through Taguchi design method. The amount of produced enzyme was measured by carbazole method.Results: According to Taguchi design method, culture medium containing fructose (5g/l) yeast extract (15g/l), ammonium chloride (10g/l) with a rotational speed (250rpm) was condition to produce the enzyme through G8. The amount of produced enzyme was very significant in such condition (8.04unit/ml).Conclusion: The obtained results from the study can be used to design any suitable industrial culture media and to determine the best physicochemical condition (aeration) for economical production of hyaluronidase through G8.
