Scientific Reports (Aug 2021)
DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death
- Stefano Bonassi,
- Marcello Ceppi,
- Peter Møller,
- Amaya Azqueta,
- Mirta Milić,
- Neri Monica,
- Gunnar Brunborg,
- Roger Godschalk,
- Gudrun Koppen,
- Sabine A. S. Langie,
- João Paulo Teixeira,
- Marco Bruzzone,
- Juliana Da Silva,
- Danieli Benedetti,
- Delia Cavallo,
- Cinzia Lucia Ursini,
- Lisa Giovannelli,
- Silvia Moretti,
- Patrizia Riso,
- Cristian Del Bo’,
- Patrizia Russo,
- Malgorzata Dobrzyńska,
- Irina A. Goroshinskaya,
- Ekaterina I. Surikova,
- Marta Staruchova,
- Magdalena Barančokova,
- Katarina Volkovova,
- Alena Kažimirova,
- Bozena Smolkova,
- Blanca Laffon,
- Vanessa Valdiglesias,
- Susana Pastor,
- Ricard Marcos,
- Alba Hernández,
- Goran Gajski,
- Biljana Spremo-Potparević,
- Lada Živković,
- Elisa Boutet-Robinet,
- Hervé Perdry,
- Pierre Lebailly,
- Carlos L. Perez,
- Nursen Basaran,
- Zsuzsanna Nemeth,
- Anna Safar,
- Maria Dusinska,
- Andrew Collins,
- for the hCOMET project
Affiliations
- Stefano Bonassi
- Unit of Clinical and Molecular Epidemiology, IRCCS San Raffaele Roma
- Marcello Ceppi
- Clinical Epidemiology Unit, San Martino Policlinic Hospital
- Peter Møller
- Department of Public Health, Section of Environmental Health, University of Copenhagen
- Amaya Azqueta
- Department of Pharmacology and Toxicology, University of Navarra
- Mirta Milić
- Mutagenesis Unit, Institute for Medical Research and Occupational Health
- Neri Monica
- Unit of Clinical and Molecular Epidemiology, IRCCS San Raffaele Roma
- Gunnar Brunborg
- Department of Environmental Health, Section of Molecular Toxicology, Norwegian Institute of Public Health (NIPH)
- Roger Godschalk
- Department of Pharmacology and Toxicology, School of Nutrition and Translational Research in Metabolism, University of Maastricht
- Gudrun Koppen
- Flemish Institute of Technological Research, Environmental Risk and Health Unit VITO - BIOMo
- Sabine A. S. Langie
- Department of Pharmacology and Toxicology, School of Nutrition and Translational Research in Metabolism, University of Maastricht
- João Paulo Teixeira
- Environmental Health Department, National Institute of Health, Rua Alexandre Herculano
- Marco Bruzzone
- Clinical Epidemiology Unit, San Martino Policlinic Hospital
- Juliana Da Silva
- Laboratory of Genetic Toxicology, Lutheran University of Brazil (ULBRA), and La Salle University (UNILASALLE)
- Danieli Benedetti
- Laboratory of Genetic Toxicology, Lutheran University of Brazil (ULBRA), and La Salle University (UNILASALLE)
- Delia Cavallo
- Department of Occupational and Environmental Medicine, Epidemiology and Hygiene (DiMEILA), Italian Workers’ Compensation Authority (INAIL)
- Cinzia Lucia Ursini
- Department of Occupational and Environmental Medicine, Epidemiology and Hygiene (DiMEILA), Italian Workers’ Compensation Authority (INAIL)
- Lisa Giovannelli
- Department NEUROFARBA, University of Florence
- Silvia Moretti
- Department of Health Sciences, Division of Dermatology, University of Florence, Palagi Hospital
- Patrizia Riso
- Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan
- Cristian Del Bo’
- Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan
- Patrizia Russo
- Unit of Clinical and Molecular Epidemiology, IRCCS San Raffaele Roma
- Malgorzata Dobrzyńska
- Department of Radiation Hygiene and Radiobiology, National Institute of Public Health NIH – National Research Institute
- Irina A. Goroshinskaya
- Laboratory for the Study of the Pathogenesis of Malignant Tumors, National Medical Research Center for Oncology
- Ekaterina I. Surikova
- Laboratory for the Study of the Pathogenesis of Malignant Tumors, National Medical Research Center for Oncology
- Marta Staruchova
- Institute of Biology, Medical Faculty, Slovak Medical University
- Magdalena Barančokova
- Institute of Biology, Medical Faculty, Slovak Medical University
- Katarina Volkovova
- Institute of Biology, Medical Faculty, Slovak Medical University
- Alena Kažimirova
- Institute of Biology, Medical Faculty, Slovak Medical University
- Bozena Smolkova
- Cancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences
- Blanca Laffon
- Grupo DICOMOSA, Centro de Investigaciones Científicas Avanzadas (CICA), Departamento de Psicología, Facultad de Ciencias de La Educación, Universidade da Coruña
- Vanessa Valdiglesias
- Instituto de Investigación Biomédica de A Coruña (INIBIC), AE CICA-INIBIC
- Susana Pastor
- Department of Genetics and Microbiology, Faculty of Biosciences, Universitat Autònoma de Barcelona
- Ricard Marcos
- Department of Genetics and Microbiology, Faculty of Biosciences, Universitat Autònoma de Barcelona
- Alba Hernández
- Department of Genetics and Microbiology, Faculty of Biosciences, Universitat Autònoma de Barcelona
- Goran Gajski
- Mutagenesis Unit, Institute for Medical Research and Occupational Health
- Biljana Spremo-Potparević
- Center of Biological Research, Faculty of Pharmacy, University of Belgrade
- Lada Živković
- Center of Biological Research, Faculty of Pharmacy, University of Belgrade
- Elisa Boutet-Robinet
- Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRAE, ENVT, INP-Purpan, UPS
- Hervé Perdry
- Univ Paris-Saclay, CESP
- Pierre Lebailly
- ANTICIPE Unit, INSERM & University of Caen-Normandie Centre François Baclesse
- Carlos L. Perez
- Department of Biochemistry, Instituto de Ciencias Básicas Y Preclínicas “Victoria de Giron”, Universidad de Ciencias Médicas de La Habana
- Nursen Basaran
- Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Hacettepe University
- Zsuzsanna Nemeth
- Department of Non-Ionizing Radiation, National Public Health Center
- Anna Safar
- Department of Non-Ionizing Radiation, National Public Health Center
- Maria Dusinska
- NILU, Health Effects Laboratory
- Andrew Collins
- Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo
- for the hCOMET project
- DOI
- https://doi.org/10.1038/s41598-021-95976-7
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 11
Abstract
Abstract The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.
