Exogenous 24-epibrassinolide ameliorates high temperature-induced inhibition of growth and photosynthesis in Cucumis melo

Abstract

This study was carried out to better understand the role of 24-epibrassinolide (EBR) in thermotolerance of melon (Cucumis melo L.). The melon seedlings were pretreated with various concentrations of EBR (0, 0.05, 0.1, 0.5, 1.0, and 1.5 mg dm-3) as foliar spray and then exposed to a high temperature (HT) stress. Exogenous EBR (0.5-1.5 mg dm-3) alleviated HT-caused growth suppression. In parallel, 1.0 mg dm-3 EBR attenuated the decrease in chlorophyll content, net photosynthetic rate, stomatal conductance, maximum quantum efficiency of photosystem (PS) II, quantum yield of PS II, and photochemical quenching of chlorophyll a fluorescence in HT-stressed plants, and inhibited transpiration rate and non-photochemical quenching. Furthermore, exogenous EBR also significantly reduced the content of malondialdehyde (MDA) and increased the content of soluble proteins and free proline, and activities of antioxidant enzymes including superoxide dismutase, guaiacol peroxidase, catalase, and ascorbate peroxidase under the HT stress. The results show that protective effects of EBR against the HT stress in the melon seedlings were most likely mediated through the improvement of photosynthesis and the stimulation of antioxidant capacity.

Keywords

• ascorbate peroxidase
• brassinosteroids
• catalase
• chlorophyll
• malondialdehyde
• melon
• net photosynthetic rate
• peroxidase
• photosystem ii
• proline
• stomatal conductance
• superoxide dismutase
• transpiration rate